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    Structured Review

    New England Biolabs band mobility
    Band Mobility, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1498 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1+kb+dna+ladder/1+kb+DNA+Ladder/pm42129646-56-6-14
    Average 97 stars, based on 1498 article reviews
    band mobility - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: SMAD2 linker phosphorylation impacts overall survival, proliferation, TGFβ1-dependent gene expression and pluripotency-related proteins in NSCLC.
    Article Snippet: .. DNA bands were detected by ethidium bromide and a 1-kb DNA ladder (New England Biolabs) in a 3% agarose gel exposed to ultraviolet light. .. We obtained two potential clones evaluated by sequencing (Eurofins Genomic, Ebersberg, Germany) using primer seqSMAD2-a_fw and seqSMAD2-a_rv.

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Purified gDNA (Support Protocol ) Nuclease‐free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067‐5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067‐5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol ) 10‐β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara‐leu) 7697 araD139 fhu A Δ lacX74 galK16 galE15 e14‐ Φ80 dlacZ Δ M15 recA1 relA1 endA1 nupG rpsL (Str R ) rph spoT1 Δ( mrr ‐ hsdRMS ‐ mcrBC ) 10‐β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris‐borate‐EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1‐kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g‐TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5‐ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1‐mm electroporation cuvettes (BTX, cat. no. 45‐0124) Gene Pulser Xcell Electroporator (Bio‐Rad, cat. no. 165‐2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612‐5496) 90‐mm petri plate (Corning, cat. no. CLS430599‐60EA) Thermocycler Apparatus and equipment for agarose gel electrophoresis 245 × 245‐mm square bioassay plate (Corning, cat. no. 431272) 50‐ml Falcon tubes (Corning, cat. no. CLS430829‐500EA) ..

    Article Title: SMAD2 linker phosphorylation impacts overall survival, proliferation, TGFβ1-dependent gene expression and pluripotency-related proteins in NSCLC
    Article Snippet: .. DNA bands were detected by ethidium bromide and a 1-kb DNA ladder (New England Biolabs) in a 3% agarose gel exposed to ultraviolet light. .. We obtained two potential clones evaluated by sequencing (Eurofins Genomic, Ebersberg, Germany) using primer seqSMAD2-a_fw and seqSMAD2-a_rv.

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Purified gDNA (Support Protocol 1) Nuclease-free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067-5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067-5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol 2) 10-β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara-leu) 7697 araD139 fhuA lacX74 galK16 galE15 e1480dlacZ M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 (mrr-hsdRMS-mcrBC) 10-β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris-borate-EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1-kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g-TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5-ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1-mm electroporation cuvettes (BTX, cat. no. 45-0124) Gene Pulser Xcell Electroporator (Bio-Rad, cat. no. 165-2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612-5496) 90-mm petri plate (Corning, cat. no. CLS430599-60EA) Thermocycler Florez-Cardona et al. 6 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. ..

    Purification:

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Purified gDNA (Support Protocol ) Nuclease‐free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067‐5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067‐5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol ) 10‐β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara‐leu) 7697 araD139 fhu A Δ lacX74 galK16 galE15 e14‐ Φ80 dlacZ Δ M15 recA1 relA1 endA1 nupG rpsL (Str R ) rph spoT1 Δ( mrr ‐ hsdRMS ‐ mcrBC ) 10‐β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris‐borate‐EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1‐kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g‐TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5‐ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1‐mm electroporation cuvettes (BTX, cat. no. 45‐0124) Gene Pulser Xcell Electroporator (Bio‐Rad, cat. no. 165‐2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612‐5496) 90‐mm petri plate (Corning, cat. no. CLS430599‐60EA) Thermocycler Apparatus and equipment for agarose gel electrophoresis 245 × 245‐mm square bioassay plate (Corning, cat. no. 431272) 50‐ml Falcon tubes (Corning, cat. no. CLS430829‐500EA) ..

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Purified gDNA (Support Protocol 1) Nuclease-free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067-5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067-5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol 2) 10-β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara-leu) 7697 araD139 fhuA lacX74 galK16 galE15 e1480dlacZ M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 (mrr-hsdRMS-mcrBC) 10-β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris-borate-EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1-kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g-TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5-ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1-mm electroporation cuvettes (BTX, cat. no. 45-0124) Gene Pulser Xcell Electroporator (Bio-Rad, cat. no. 165-2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612-5496) 90-mm petri plate (Corning, cat. no. CLS430599-60EA) Thermocycler Florez-Cardona et al. 6 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. ..

    Magnetic Beads:

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Purified gDNA (Support Protocol ) Nuclease‐free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067‐5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067‐5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol ) 10‐β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara‐leu) 7697 araD139 fhu A Δ lacX74 galK16 galE15 e14‐ Φ80 dlacZ Δ M15 recA1 relA1 endA1 nupG rpsL (Str R ) rph spoT1 Δ( mrr ‐ hsdRMS ‐ mcrBC ) 10‐β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris‐borate‐EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1‐kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g‐TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5‐ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1‐mm electroporation cuvettes (BTX, cat. no. 45‐0124) Gene Pulser Xcell Electroporator (Bio‐Rad, cat. no. 165‐2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612‐5496) 90‐mm petri plate (Corning, cat. no. CLS430599‐60EA) Thermocycler Apparatus and equipment for agarose gel electrophoresis 245 × 245‐mm square bioassay plate (Corning, cat. no. 431272) 50‐ml Falcon tubes (Corning, cat. no. CLS430829‐500EA) ..

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Purified gDNA (Support Protocol 1) Nuclease-free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067-5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067-5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol 2) 10-β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara-leu) 7697 araD139 fhuA lacX74 galK16 galE15 e1480dlacZ M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 (mrr-hsdRMS-mcrBC) 10-β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris-borate-EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1-kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g-TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5-ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1-mm electroporation cuvettes (BTX, cat. no. 45-0124) Gene Pulser Xcell Electroporator (Bio-Rad, cat. no. 165-2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612-5496) 90-mm petri plate (Corning, cat. no. CLS430599-60EA) Thermocycler Florez-Cardona et al. 6 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. ..

    Plasmid Preparation:

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Purified gDNA (Support Protocol ) Nuclease‐free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067‐5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067‐5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol ) 10‐β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara‐leu) 7697 araD139 fhu A Δ lacX74 galK16 galE15 e14‐ Φ80 dlacZ Δ M15 recA1 relA1 endA1 nupG rpsL (Str R ) rph spoT1 Δ( mrr ‐ hsdRMS ‐ mcrBC ) 10‐β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris‐borate‐EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1‐kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g‐TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5‐ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1‐mm electroporation cuvettes (BTX, cat. no. 45‐0124) Gene Pulser Xcell Electroporator (Bio‐Rad, cat. no. 165‐2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612‐5496) 90‐mm petri plate (Corning, cat. no. CLS430599‐60EA) Thermocycler Apparatus and equipment for agarose gel electrophoresis 245 × 245‐mm square bioassay plate (Corning, cat. no. 431272) 50‐ml Falcon tubes (Corning, cat. no. CLS430829‐500EA) ..

    Article Title: Dual roles of EGO-1 and RRF-1 in regulating germline exo-RNAi efficiency in Caenorhabditis elegans
    Article Snippet: .. We injected a genome editing plasmid mixture containing two Cas9/sgRNA plasmids targeting rrf-1 (25 ng/μl each), an injection marker plasmid pCFJ90 (Pmyo-2::mCherry) (5 ng/μl), and an NEB 1 kb DNA ladder (145 ng/μl) into the gonads of wild-type or ego-1(gk532049) animals. ..

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Purified gDNA (Support Protocol 1) Nuclease-free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067-5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067-5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol 2) 10-β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara-leu) 7697 araD139 fhuA lacX74 galK16 galE15 e1480dlacZ M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 (mrr-hsdRMS-mcrBC) 10-β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris-borate-EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1-kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g-TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5-ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1-mm electroporation cuvettes (BTX, cat. no. 45-0124) Gene Pulser Xcell Electroporator (Bio-Rad, cat. no. 165-2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612-5496) 90-mm petri plate (Corning, cat. no. CLS430599-60EA) Thermocycler Florez-Cardona et al. 6 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. ..

    Extraction:

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Purified gDNA (Support Protocol ) Nuclease‐free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067‐5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067‐5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol ) 10‐β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara‐leu) 7697 araD139 fhu A Δ lacX74 galK16 galE15 e14‐ Φ80 dlacZ Δ M15 recA1 relA1 endA1 nupG rpsL (Str R ) rph spoT1 Δ( mrr ‐ hsdRMS ‐ mcrBC ) 10‐β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris‐borate‐EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1‐kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g‐TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5‐ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1‐mm electroporation cuvettes (BTX, cat. no. 45‐0124) Gene Pulser Xcell Electroporator (Bio‐Rad, cat. no. 165‐2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612‐5496) 90‐mm petri plate (Corning, cat. no. CLS430599‐60EA) Thermocycler Apparatus and equipment for agarose gel electrophoresis 245 × 245‐mm square bioassay plate (Corning, cat. no. 431272) 50‐ml Falcon tubes (Corning, cat. no. CLS430829‐500EA) ..

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Purified gDNA (Support Protocol 1) Nuclease-free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067-5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067-5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol 2) 10-β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara-leu) 7697 araD139 fhuA lacX74 galK16 galE15 e1480dlacZ M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 (mrr-hsdRMS-mcrBC) 10-β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris-borate-EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1-kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g-TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5-ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1-mm electroporation cuvettes (BTX, cat. no. 45-0124) Gene Pulser Xcell Electroporator (Bio-Rad, cat. no. 165-2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612-5496) 90-mm petri plate (Corning, cat. no. CLS430599-60EA) Thermocycler Florez-Cardona et al. 6 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. ..

    Spectrophotometry:

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Purified gDNA (Support Protocol ) Nuclease‐free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067‐5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067‐5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol ) 10‐β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara‐leu) 7697 araD139 fhu A Δ lacX74 galK16 galE15 e14‐ Φ80 dlacZ Δ M15 recA1 relA1 endA1 nupG rpsL (Str R ) rph spoT1 Δ( mrr ‐ hsdRMS ‐ mcrBC ) 10‐β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris‐borate‐EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1‐kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g‐TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5‐ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1‐mm electroporation cuvettes (BTX, cat. no. 45‐0124) Gene Pulser Xcell Electroporator (Bio‐Rad, cat. no. 165‐2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612‐5496) 90‐mm petri plate (Corning, cat. no. CLS430599‐60EA) Thermocycler Apparatus and equipment for agarose gel electrophoresis 245 × 245‐mm square bioassay plate (Corning, cat. no. 431272) 50‐ml Falcon tubes (Corning, cat. no. CLS430829‐500EA) ..

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Purified gDNA (Support Protocol 1) Nuclease-free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067-5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067-5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol 2) 10-β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara-leu) 7697 araD139 fhuA lacX74 galK16 galE15 e1480dlacZ M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 (mrr-hsdRMS-mcrBC) 10-β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris-borate-EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1-kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g-TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5-ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1-mm electroporation cuvettes (BTX, cat. no. 45-0124) Gene Pulser Xcell Electroporator (Bio-Rad, cat. no. 165-2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612-5496) 90-mm petri plate (Corning, cat. no. CLS430599-60EA) Thermocycler Florez-Cardona et al. 6 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. ..

    Polymerase Chain Reaction:

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Purified gDNA (Support Protocol ) Nuclease‐free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067‐5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067‐5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol ) 10‐β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara‐leu) 7697 araD139 fhu A Δ lacX74 galK16 galE15 e14‐ Φ80 dlacZ Δ M15 recA1 relA1 endA1 nupG rpsL (Str R ) rph spoT1 Δ( mrr ‐ hsdRMS ‐ mcrBC ) 10‐β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris‐borate‐EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1‐kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g‐TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5‐ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1‐mm electroporation cuvettes (BTX, cat. no. 45‐0124) Gene Pulser Xcell Electroporator (Bio‐Rad, cat. no. 165‐2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612‐5496) 90‐mm petri plate (Corning, cat. no. CLS430599‐60EA) Thermocycler Apparatus and equipment for agarose gel electrophoresis 245 × 245‐mm square bioassay plate (Corning, cat. no. 431272) 50‐ml Falcon tubes (Corning, cat. no. CLS430829‐500EA) ..

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Purified gDNA (Support Protocol 1) Nuclease-free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067-5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067-5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol 2) 10-β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara-leu) 7697 araD139 fhuA lacX74 galK16 galE15 e1480dlacZ M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 (mrr-hsdRMS-mcrBC) 10-β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris-borate-EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1-kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g-TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5-ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1-mm electroporation cuvettes (BTX, cat. no. 45-0124) Gene Pulser Xcell Electroporator (Bio-Rad, cat. no. 165-2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612-5496) 90-mm petri plate (Corning, cat. no. CLS430599-60EA) Thermocycler Florez-Cardona et al. 6 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. ..

    Electroporation:

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Purified gDNA (Support Protocol ) Nuclease‐free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067‐5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067‐5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol ) 10‐β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara‐leu) 7697 araD139 fhu A Δ lacX74 galK16 galE15 e14‐ Φ80 dlacZ Δ M15 recA1 relA1 endA1 nupG rpsL (Str R ) rph spoT1 Δ( mrr ‐ hsdRMS ‐ mcrBC ) 10‐β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris‐borate‐EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1‐kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g‐TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5‐ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1‐mm electroporation cuvettes (BTX, cat. no. 45‐0124) Gene Pulser Xcell Electroporator (Bio‐Rad, cat. no. 165‐2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612‐5496) 90‐mm petri plate (Corning, cat. no. CLS430599‐60EA) Thermocycler Apparatus and equipment for agarose gel electrophoresis 245 × 245‐mm square bioassay plate (Corning, cat. no. 431272) 50‐ml Falcon tubes (Corning, cat. no. CLS430829‐500EA) ..

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Purified gDNA (Support Protocol 1) Nuclease-free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067-5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067-5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol 2) 10-β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara-leu) 7697 araD139 fhuA lacX74 galK16 galE15 e1480dlacZ M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 (mrr-hsdRMS-mcrBC) 10-β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris-borate-EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1-kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g-TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5-ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1-mm electroporation cuvettes (BTX, cat. no. 45-0124) Gene Pulser Xcell Electroporator (Bio-Rad, cat. no. 165-2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612-5496) 90-mm petri plate (Corning, cat. no. CLS430599-60EA) Thermocycler Florez-Cardona et al. 6 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. ..

    Sterility:

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Purified gDNA (Support Protocol ) Nuclease‐free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067‐5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067‐5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol ) 10‐β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara‐leu) 7697 araD139 fhu A Δ lacX74 galK16 galE15 e14‐ Φ80 dlacZ Δ M15 recA1 relA1 endA1 nupG rpsL (Str R ) rph spoT1 Δ( mrr ‐ hsdRMS ‐ mcrBC ) 10‐β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris‐borate‐EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1‐kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g‐TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5‐ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1‐mm electroporation cuvettes (BTX, cat. no. 45‐0124) Gene Pulser Xcell Electroporator (Bio‐Rad, cat. no. 165‐2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612‐5496) 90‐mm petri plate (Corning, cat. no. CLS430599‐60EA) Thermocycler Apparatus and equipment for agarose gel electrophoresis 245 × 245‐mm square bioassay plate (Corning, cat. no. 431272) 50‐ml Falcon tubes (Corning, cat. no. CLS430829‐500EA) ..

    Article Title: Plasmid Library Construction From Genomic DNA.
    Article Snippet: .. Purified gDNA (Support Protocol 1) Nuclease-free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067-5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067-5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol 2) 10-β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara-leu) 7697 araD139 fhuA lacX74 galK16 galE15 e1480dlacZ M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 (mrr-hsdRMS-mcrBC) 10-β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris-borate-EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1-kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g-TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5-ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13-400-525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1-mm electroporation cuvettes (BTX, cat. no. 45-0124) Gene Pulser Xcell Electroporator (Bio-Rad, cat. no. 165-2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612-5496) 90-mm petri plate (Corning, cat. no. CLS430599-60EA) Thermocycler Florez-Cardona et al. 6 of 24 Current Protocols 26911299, 2025, 1, D ow nloaded from https://currentprotocols.onlinelibrary.w iley.com /doi/10.1002/cpz1.70088 by IN A SP - N E PA L , W iley O nline L ibrary on [26/01/2025]. ..

    Bioassay:

    Article Title: Plasmid Library Construction From Genomic DNA
    Article Snippet: .. Purified gDNA (Support Protocol ) Nuclease‐free water (NEB, cat. no. B1500S) Bioanalyzer Genomic DNA ScreenTape assay kit (Agilent Technologies, cat. no. 5067‐5365) Bioanalyzer Genomic DNA ScreenTape reagents: Ladder and Loading Buffer (Agilent Technologies, cat. no. 5067‐5366) Qubit reagents: 1× dsDNA BR working solution (Invitrogen, cat. no. Q33262) Qubit 1× dsDNA BR Standard #1 and #2 (Invitrogen, cat. no. Q33263) Quick Blunting Kit (NEB, cat. no. E1201S) 95% ethanol AMPure magnetic beads (Beckman Coulter, cat. no. A63880) Blunt/TA Ligase Master Mix (NEB, cat. no. M0367S) Linearized, purified vector (Support Protocol ) 10‐β competent E. coli (high efficiency; NEB, cat. no. C3020S), genotype E. coli DHB10 Δ(ara‐leu) 7697 araD139 fhu A Δ lacX74 galK16 galE15 e14‐ Φ80 dlacZ Δ M15 recA1 relA1 endA1 nupG rpsL (Str R ) rph spoT1 Δ( mrr ‐ hsdRMS ‐ mcrBC ) 10‐β/Stable Outgrowth Medium (NEB, cat. no. B9035S) LB agar with appropriate antibiotic(s) Q5 High Fidelity 2× Master Mix (NEB, cat. no. 0492S) Appropriate primers 1% agarose gel (Lonza, cat. no. 54905) 5× Tris‐borate‐EDTA (TBE) buffer (Thermo Fisher, cat. no. J63487.3) 1‐kb DNA ladder (NEB, cat. no. N3232L) 6× gel loading dye, purple (NEB, cat. no. B7024S) Plasmid Midiprep Extraction Kit (100; Qiagen, cat. no. 12145) g‐TUBES (Covaris, cat. no. 520079) Tabletop centrifuge (Eppendorf 5424, cat. no. 5404000014) Bioanalyzer equipment (Agilent Technologies, cat. no. G2991BA) Qubit 4 fluorometer (Invitrogen, cat. no. Q33238) 1.5‐ml tubes (Eppendorf, cat. no. 0030123611) NanoDrop spectrophotometer (Thermo Fisher, cat. no. 13‐400‐525) Magnetic rack (NEB, cat. no. S1509S) Minicentrifuge (Thermo Fisher, cat. no. 75004061) PCR tubes (Eppendorf, cat. no. 951010006) 1‐mm electroporation cuvettes (BTX, cat. no. 45‐0124) Gene Pulser Xcell Electroporator (Bio‐Rad, cat. no. 165‐2100) Incubator (Eppendorf, cat. no. S44I300001) Sterile cell spreader (VWR, cat. no. 612‐5496) 90‐mm petri plate (Corning, cat. no. CLS430599‐60EA) Thermocycler Apparatus and equipment for agarose gel electrophoresis 245 × 245‐mm square bioassay plate (Corning, cat. no. 431272) 50‐ml Falcon tubes (Corning, cat. no. CLS430829‐500EA) ..

    Injection:

    Article Title: Dual roles of EGO-1 and RRF-1 in regulating germline exo-RNAi efficiency in Caenorhabditis elegans
    Article Snippet: .. We injected a genome editing plasmid mixture containing two Cas9/sgRNA plasmids targeting rrf-1 (25 ng/μl each), an injection marker plasmid pCFJ90 (Pmyo-2::mCherry) (5 ng/μl), and an NEB 1 kb DNA ladder (145 ng/μl) into the gonads of wild-type or ego-1(gk532049) animals. ..

    Marker:

    Article Title: Dual roles of EGO-1 and RRF-1 in regulating germline exo-RNAi efficiency in Caenorhabditis elegans
    Article Snippet: .. We injected a genome editing plasmid mixture containing two Cas9/sgRNA plasmids targeting rrf-1 (25 ng/μl each), an injection marker plasmid pCFJ90 (Pmyo-2::mCherry) (5 ng/μl), and an NEB 1 kb DNA ladder (145 ng/μl) into the gonads of wild-type or ego-1(gk532049) animals. ..

    other:

    Article Title: Protocol for generating splice isoform-specific mouse mutants using CRISPR-Cas9 and a minigene splicing reporter
    Article Snippet: 1 kb DNA ladder , New England Biolabs , N3232L.



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    Journal: Poultry Science

    Article Title: Construction and modification of a low-copy plasmid-based infectious clone for GI-19 genotype IBV via Red/ET recombineering: A simplified and efficient reverse genetics system for co ronavirus

    doi: 10.1016/j.psj.2026.106881

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    Article Snippet: M: TaKaRa 1 kb DNA Ladder.

    Techniques: Biomarker Discovery, Recombinant, Functional Assay, Selection, Generated, Software, Electrophoresis, Staining, Marker, Plasmid Preparation, Transformation Assay, Bacteria